North America elatus fingerprint and with Oplopanax A Comparative Study of chemical constituents of ginseng.doc

North America elatus fingerprint and with Oplopanax A Comparative Study of chemical constituents of ginseng Author: Zhao-yin, contin States, De-Qiang Dou, Zhang Fang, Wang’s first 1000 [Abstract] Objective To study the North American elatus (Oplopanax horridus, OH) of the HPLC fingerprint for the scientific evaluation and effective control of the quality of herbs to provide a reliable method. Methods for the reference substance syringin established O.horridus bark HPLC fingerprints, chromatographic conditions: column Kromasil C18 column (250 mm * 4.6 mm, 5 μ m), pre-column Zorbax (12.5 mm * 4.6 mm, 5 μ m) mobile phase of acetonitrile -0.05% phosphoric acid gradient elution flow rate was 1.0 ml / min, column temperature 35 ℃ , detection wavelength 210 nm. Of the 11 batches of bark medicines were similarity analysis, and with the Northeast elatus (O. elatus Nakai, OE) bark fingerprints were compared, using cluster analysis method to process data. Result is the same type of herbs similarity higher than 0.9, using cluster analysis can be clustered into one class. Conclusion elatus fingerprint can be used to identify different types of medicine. [Keywords:] North America elatus; Oplopanax ginseng; fingerprinting; cluster analysis AbstractObjectiveTo study on the HPLC fingerprints of Oplopanax horridus for offering a method on the quality control and assessment. MethodsSyringenin was used as reference substance to establish HPLC fingerprints of stem bark of O. horridus. Separations and determinations were carried out by linear gradient elution under following conditions: Kromasil C18 column (250 mm * 4.6 mm, 5 μ m), pre-column Zorbax (12.5 mm * 4.6 mm, 5 μ m), acetonitrile-0.05% phosphoric acid solution as mobile phase, detection wavelength of UV at 210nm, flow -rate of 1.0 ml / min, column temperature of 35 ℃ . Similarity calculation software was used to evaluate the whole HPLC spectrums of 11 samples of OH stem bark. Furthermore, it was compared with fingerprint o