Non-small cell lung cancer patients Detection of serum protein markers.doc

 PAGE \* MERGEFORMAT 12 Non-small cell lung cancer patients Detection of serum protein markers Of: Yang Yang Zhao Song Jianjun Liu Dong Tang Yan Lei Zhu [Abstract] Objective To detect non-small cell lung cancer (NSCLC serum protein, specific protein markers selected to build for the early diagnosis of NSCLC serum protein fingerprint model. Methods surface-enhanced laser desorption ionization time of flight mass spectrometry (SELDI TOF MS) serum samples of 235 cases detected by mass spectrometry of proteins, combined with bioinformatics methods (support vector machine) to analyze the data. The results were screened 3 z (m / z) in 6628,9191 and 11412 protein marker, building NSCLC early diagnosis. United 3 potential protein markers, the leave-one-out cross validation, the distinction between NSCLC and normal healthy controls with a sensitivity of 98%, a specificity of 96%. blind test shows that the model sensitivity of diagnosis of NSCLC was 96.56%, a specificity of 94.79%. Conclusion SELDI TOF MS combined with support vector machine model of NSCLC serum protein pattern of early diagnosis of NSCLC, a high sensitivity and specificity of the new method is worthy of further research and application. [Keywords:] non-small cell lung cancer, diagnosis, support vector machines, biological markers Lung cancer is the world’s highest death rates in tumors, all cancer deaths accounted for about 25% (1). Non-small cell lung cancer (NSCLC accounts for about 80% incidence of lung cancer (2). Early diagnosis and reasonable treatment to improve long-term survival and prognosis of NSCLC patients is important (3). NSCLC stage mainly depends on the clinical diagnosis of chest X ray camera, CT, cell puncture study, bronchoscopy, etc., although most cases can be diagnosed, but not early detection and prognosis of missed timing of treatment effects (4). proteomics for the early diagnosis of NSCLC provides new ideas and technology platform (5). In this study, surface-enhanc