Nucleic acid sequence-based amplification technology in the allogeneic peripheral blood stem cell transplant recipients after the diagnosis of HCMV infection in.doc

 PAGE \* MERGEFORMAT 8 Nucleic acid sequence-based amplification technology in the allogeneic peripheral blood stem cell transplant recipients after the diagnosis of HCMV infection in Author: Xu Hongxu Liu Yafeng Tan Xun Cai Jiantao Abstract This study investigated the nucleic acid sequence-based amplification (nucleic acid sequence based amplification, NASBA) detection of mRNA for allogeneic peripheral blood stem cell transplant recipients after human cytomegalovirus (HCMV) infection in the diagnosis and to evaluate the Technical guide for antiviral therapy. To 128 cases of transplantation patients were studied, collected 352 peripheral blood after transplantation; the use of NASBA detected HCMV in peripheral blood matrix coat protein PP67 (UL65) mRNA, and with the HCMV DNA results were compared. The results showed that: 352 were blood samples, HCMV mRNA-positive 105 (29.83%), HCMV DNA-positive 183 (51.99%). 128 patients after 45 patients developed HCMV disease, HCMV DNA and HCMV mRNA detection of HCMV disease diagnostic sensitivity and specificity were 95.56% (43/45), 93.33% (42/45) and 60.24% (50 / 83), 97.59% (81/83). Specific difference between the two was significant (P lt;0.05). Conclusion: HCMV-PP67 in NASBA detection method is a rapid, high sensitivity and specificity, etc., can be used as rapid detection methods for clinical HCMV disease. The assay results with transplantation-related clinical symptoms, it can also be used to monitor allogeneic hematopoietic stem cell transplant recipients after HCMV infection and to guide anti-viral treatment basis. Keywords: allogeneic peripheral blood stem cell transplantation of human cytomegalovirus infection in nucleic acid sequence-based amplification Application of Nucleic Acid Sequence-Based Amplification in Detection of Cytomegalovirus mRNA of Recipients after Allogeneic Peripheral Blood Stem Cell Transplantation AbstractThe study was purposed to investigate diagnostic valu