O-phthalaldehyde pre-column derivatization reversed-phase high performance liquid chromatography of amino acids in pearl powder.doc

 PAGE \* MERGEFORMAT 11 O-phthalaldehyde pre-column derivatization reversed-phase high performance liquid chromatography of amino acids in pearl powder Author: Chen Zhi, Chen Bo, Jie Xin-Ming, Zhang Jian 【Abstract】 Objective To determine the amino acid content of pearl powder. Methods o-phthalaldehyde (OPA) pre-column derivatization RP-HPLC method with fluorescence detection, gradient elution. The results of the Act, within 28 min in the isolated 15 kinds of amino acids. Conclusion This method is to use less sample, high sensitivity, wide linear range, good reproducibility and is suitable for separation of a large number of samples. Keywords: high-performance liquid chromatography; pre-column derivatization method; amino acids; pearl powder Determination of the Contents of Amino Acids in Pearl Powder by RP-HPLC after Pre  column Derivation with O  Phthaldialdehyde (OPA) Abstract: ObjectiveIn this paper, we determined amino acids in pearl powder. Methods A manual pre-column derivation procedure has been developed by reversed-phase HPLC with O-phthalaldehyde and fluorescence detector. Results More than 15 amino acids were separated within 28 min. Conclusion The method is sensitive, precise and shows good repeatability. Keywords:: High  performance liquid chromatography; Pre  column derivation; Amino acids; Pearl powder With the life sciences and bio-engineering development, amino acid composition of biological macromolecules as the basic unit of the analysis methods be taken seriously. The 20th century, 70 years later, reversed-phase high performance liquid chromatography in the amino acid analysis has been widely used to shorten the separation time. While the fluorescent pre-column derivatization technology enables improved detection sensitivity of two orders of magnitude, reaching pmol level. Derivative methods are commonly used: DABS method (derived agent is chloride dimethylamino azobenzene  DABSYL  Cl),