Of chlorogenic acid in chrysanthemum Extraction Process.docVIP

 PAGE \* MERGEFORMAT 8 Of chlorogenic acid in chrysanthemum Extraction Process [Abstract] Objective Optimization of chlorogenic acid in chrysanthemum the extraction process. Orthogonal experiment designed to chlorogenic acid content of the indexes on the extraction process optimization. The results of the optimal water bath extraction process as follows: liquid to solid ratio of 12:1 of 70% ethanol at 80 ℃ water bath extraction 40 min. Under these conditions, chlorogenic acid content to 0.35%. The best ultrasonic extraction conditions were: liquid-solid ratio of 15:1 of 60% ethanol pre-soaking 40 min, ultrasonic extraction 45 min. Under these conditions, chlorogenic acid content to 0.36%. Ultrasound method is better than water bath method. Conclusion of chlorogenic acid in the extraction process to ensure a high mass fraction of chlorogenic acid to optimize the wild chrysanthemum in the extraction process of chlorogenic acid. [Keywords:] wild chrysanthemum; chlorogenic acid; orthogonal experiment; spectrophotometry; Extraction Process Abstract: ObjectiveTo obtain optimum extraction technology of chlorogenic acid from Flos Chrysanthemi Indici.MethodsThe extraction processes for chlorogenic acid from Flos Chrysanthemi Indici. With water bath extraction and ultrasonic wave were investigated using the content of chlorogenic acid as index. The optimal extraction conditions were determined with orthogonal design. ResultsFor water bath extraction, F. Chrysanthemi Indici was extracted with 12 times the mass of 70% ethanol and refluxed at 80 ℃ for 40 min. The content of flavonoids was 0.35%. For ultrasonic wave extraction, F. Chrysanthemi Indici was extracted with 15 times the mass of 60% ethanol perimpregnating for 40 min and extracted for 45 min. The content of flavonoids was 0.35%. ConclusionThe processing method is feasible. Keywords:: F. Chrysanthemi Indici.; Chlorogenic acid; Orthogonal design; Spectrophotometry; Extracting technology Wild chrysanthemum (