Of nitric oxide synthase and atrial natriuretic peptide distribution in guinea pig cochlea.doc

 PAGE \* MERGEFORMAT 9 Of nitric oxide synthase and atrial natriuretic peptide distribution in guinea pig cochlea [Keywords:] atrial natriuretic peptide;, nitric oxide synthase;, immunofluorescence double staining [Abstract] Objective: To observe the guinea pig cochlea Local atrial natriuretic peptide (ANP) and nitric oxide synthase (NOS) immunohistochemical reaction product distribution and the relationship between ANP and NOS to study regional blood flow in guinea pig cochlea, lymphatic and nervous regulation of the interaction of the basis of morphological studies. Methods: Immunofluorescence histochemical double-labeled ANP and NOS were observed in the normal distribution of guinea pig cochlea. Results: In the cochlear spiral arteries and blood vessels of various transfer patterns were found in ANP and NOS double-positive expression, spiral limbus, spiral ligament, and Corti devices, cochlear ganglion cells ANP and NOS double-positive staining. Conclusion: ANP and NOS distribution characteristics shown, both in the inner ear blood flow regulation, inside and outside the lymph balance regulation and neural signaling may exist in such close interaction. [Keywords:] atrial natriuretic peptide; a nitric oxide synthase; immunofluorescence double staining Pairs of nitric oxide (nitric oxide, NO) research has shown that as a powerful vasodilator factor, present in the guinea pig vascular endothelium, ganglion cells and hair cells, through direct activation of ATP-sensitive potassium ion ( KATP) channel so that guinea pig spiral modiolar artery (spiral modiolar artery, SMA) endothelial and smooth muscle cells in a concentration-dependent depolarization, causing blood vessels to dilate [1]. NO key enzyme in the synthesis of nitric oxide synthase (nitric oxide synthase, NOS) is mainly distributed on the inside and outside the hair cells, cochlear ganglion cells and stria vascularis [1]. Atrial natriuretic peptide (atrial natriuretic peptide, ANP) also has a stron