Of cefotaxime and sulbactam compound preparation and in vivo antibacterial activity of.doc

 PAGE \* MERGEFORMAT 2 Of cefotaxime and sulbactam compound preparation and in vivo antibacterial activity of Author: Yang Wenji Shao-jun ZENG Yun-Gang Luo Zhou Liming [Abstract] Objective To evaluate the cefotaxime / sulbactam (CTX / SB, 1:1,2:1,4:1,8:1,16:1) and single-component cefotaxime sodium (CTX) pairs of 13 β  lactamase producing gram-negative bacteria outside the antibacterial effect, and with the compound preparation cefoperazone sodium / sulbactam sodium (CPZ / SB 1:1), and piperacillin / tazobactam (PIPC / TB 8:1) were compared, as well as producing Escherichia coli and Pseudomonas aeruginosa infection in mice protection. Methods A two-fold agar dilution method CTX / SB (1:1,2:1,4:1,8:1,16:1) compound on β  lactamase producing gram-negative bacteria and its effects on antibacterial producing Escherichia coli and Pseudomonas aeruginosa infection in mice and protective effect; biological determination of β  lactamase antibiotics relative hydrolysis rate and enzyme inhibition rate of protection, PCR amplification and detection of its drug-resistant enzymeType . Results of CTX / SB (1:1) of MICs compared with CTX in varying degrees of decline; the different ratio of CTX / SB relative hydrolysis rates were significantly lower than CTX alone (P