Of sodium arsenite on heme oxygenase -1 induced.docVIP

 PAGE \* MERGEFORMAT 4 Of sodium arsenite on heme oxygenase -1 induced [Abstract] Objective To observe the different exposure dose and time, the sodium arsenite (NaAsO2) on cultured bovine aortic endothelial cells (BAEC) in the heme oxygenase -1 (HO-1) induced by. Methods In the 0 ~ 15μ mol / L NaAsO2 exposed to 0 ~ 24h collection of cultured BAEC cell protein extract in Western blot (western blot) to detect induction of HO-1 protein generation. Results 0 ~ 15μ mol / L NaAsO2 exposure can significantly induce HO-1 protein in BAEC cells, the effect of increasing, and were in a dose - response and time - response relationship; 2  5μ mol / L NaAsO2 exposure can be markedly induced HO-1 protein. Conclusion HO-1 protein showed that arsenic-mediated induction of a cellular stress response; HO-1 protein can be used as the sensitivity of inorganic arsenic exposure biomarker, one conducive to in-depth study of inorganic arsenic exposure to the biological dose-response. [Keywords:] sodium arsenite Chronic arsenic poisoning, arsenic can cause skin damage, peripheral vascular and cardiovascular diseases and cancer, skin cancer and a variety of visceral 〔 1〕 . Heme oxygenase (HO) is the rate-limiting enzyme heme degradation. Its isozyme HO-1 is very sensitive to oxidative stress, can be a variety of stimulating and wide range of stress factors induce 〔 2〕 . The induction was considered a variety of oxidative stress in the body to stimulate an adaptive protection response. While inorganic arsenic exposure can cause a variety of cultured cells, and human and experimental animals raise the level of oxidative stress 〔 3〕 . In this study, cultured bovine aortic endothelial cells (BAEC), of sodium arsenite (NaAsO2) on the induction of HO-1. 1 Materials and methods 1  1 Main reagents and instruments sodium arsenite (NaAsO2, Japan Wako Corporation); fetal calf serum culture medium (DMEM-F12, USA Sigma Company); fibroblast growth factor (U.S. Proprotech companies); anti-HO-