Of ethanol on mouse primary cultured hepatocytes of fatty acid oxidation rate of.docVIP

 PAGE \* MERGEFORMAT 7 Of ethanol on mouse primary cultured hepatocytes of fatty acid oxidation rate of [Abstract] Objective To establish a mouse liver cells in vitro method of simultaneous detection of ethanol on liver cells, fatty acid β -oxidation rate effects. Methods pentobarbital anesthesia in mice by liver perfusion and collagenase digestion and separation of mouse liver cells were inoculated on collagen-coated Petri dishes were cultured in vitro. By HE staining cells in the growth of the state. In 20mmol / L of ethanol in the presence of, by 3H labeled palmitic acid (C16: 0) detection of liver cells, fatty acid oxidation rate. Succeeded in dividing the liver cells, liver cells, the survival rate of ≥ 95%. 48h liver cells in vitro are still able to maintain a good growth of the state. Fatty acid oxidation rate of liver cells, the study found, 20mmol / L of alcohol can lead to liver cells, fatty acid oxidation rate of about 40% lower, resulting in increased fat content of liver cells. Conclusions This study has successfully established in vitro liver cell culture method, using 3H-fatty acids in fatty acid oxidation can be sensitive to detect the speed, while the study found that ethanol can inhibit fatty acid oxidation rate of liver cells. Meanwhile, the results of this study liver cells of fat and carbohydrate metabolism study provides the experimental conditions. [Keywords:] ethanol; liver cells; fatty acids The effect of ethanol on fatty acid oxidation of mice primary hepatocytes [Abstract] Objective To isolate the mice primary hepatocytes, and the cells were cultured in vitro.The influence of ethanol on fatty acid β -oxidation velocity was measured.Methods The mouse was anesthetized with pentobarbital, and the liver was degested with collagenase.The primary hepatocytes were inoculated in collagen-coated dishes.The morphology of cultured primary hepatocytes was observed by HE staining.The fatty acid β -oxidation was measured using [9,10-3H