Of tea polyphenols on microsatellite instability in colorectal cancer in nude mice of anti-tumor effect and mechanism of.docVIP

 PAGE \* MERGEFORMAT 10 Of tea polyphenols on microsatellite instability in colorectal cancer in nude mice of anti-tumor effect and mechanism of Author: Dai Gong Jian, Jin Black Hawk, Ding Jiang, Xia Jianguo, LIU Xiu-fang, LIU Fei, Tan Xuan Zhong, Geng Xiang [Abstract] Objective: To explore the tea polyphenols on microsatellite instability in colorectal cancer nude mice and its mechanism. Methods: hMSH2 missing colon cancer cell lines Lovo cells to establish subcutaneous tumor model in nude mice, 4 weeks after inoculation using the subcutaneous tumors established colostomy orthotopic transplantation tumor model of colon cancer. 40 model in nude mice were divided into model group and 50,75,100 mg / kg polyphenols group. Intraperitoneal injection of TP treatment 5 d after the calculation of tumor inhibition rate, and detection of tumor microsatellite instability and transforming growth factor-β 1 (transforming growth factor  β 1, TGF  β 1), TGF  β 2 and insulin-like growth factor (insulin  like growth factor, IGF) fragment changes. Results: 50,75 and 100 mg / kg 3 doses of tea polyphenols after treatment, in the 1,2,3 and 4 weekend, the treatment group and model group, tumor inhibition rate, the difference was statistically significant (P amp;lt;0.01) ; comparison between the three treatment groups, no statistically significant difference. The use of tea polyphenols after treatment tumor microsatellite markers stabilized. Conclusion: TP is right or wrong mismatch repair gene deletion significantly inhibited colorectal cancer, and its mechanism may be related to inhibiting the micro-satellite instability. [Keywords:] tea polyphenols; DNA mismatch repair; gene deletion; colorectal cancer; microsatellite instability; nude mice Methods: A colostomy was performed on the caecum of nude mice. Tumor fragments collected from the subcutaneous tumor of hMSH2  absence colon carcinoma Lovo cell line were surgically implanted onto the submucosa of the caecum