OK432 MyD88 in dendritic cells maturation.docVIP

 PAGE \* MERGEFORMAT 14 OK432 MyD88 in dendritic cells maturation Of: Nixiu Xiong Liu Liyan Liu Jian Chen was really Yuanming Zhou Xuan [Abstract] Objective To investigate bone marrow dendritic cells (DCs) myeloid differentiation protein 88 (MyD88) in OK 432 induced DCs maturation. METHODS isolated from mouse bone marrow mononuclear cells (BMMCs), with 10% fetal bovine serum, recombinant mouse granulocyte macrophage colony stimulating factor (rmGM CSF), recombinant mouse interleukin 4 (rmIL 4) of the culture system induced culture, the cells obtained is the DCs. the first 5 days in culture , the cells were divided into 3 groups: control group without OK 432 and MyD88 siRNA; OK 432 group added to a final concentration of 5 g / mL of OK 432; RNA interference group joined MyD88 siRNA 12 h, then add 5 g / mL OK 432 stimuli. Semi-quantitative RT PCR detected the expression of DCs in MyD88; ELISA method to detect the DCs secrete tumor necrosis factor-@ (TNF @) and the ability of IL 12; MTT assay were mixed with DCs in vitro stimulation of immune cell proliferation activity. Results OK 432 high expression group of DCs, MyD88, TNF @, and IL 12 release was significantly increased and could induce a strong mixed lymphocyte response, and interference with MyD88 siRNA effects were lower than after (P lt;0.05). Conclusions targeting MyD88 siRNA can inhibit the expression of mouse DCs in MyD88 and OK 432-induced mature DCs, suggesting that MyD88-mediated induction of DCs maturation OK 432. [Keywords:] dendritic cell; RNA, small interfering; completion of West Barney; immune tolerance Dendritic cells (dendritic cells, DCs) in vivo is currently the most powerful known antigen presenting cells (antigen presenting cell, APC), can significantly stimulate the initial proliferation of T cells and activate the immune response and induction of immune tolerant dual function. OK 432 GMP class immunomodulator as safe and effective to improve the survival rate of patients wit