On Determination of Ephedrine Theophylline compound theobromine content of the film.docVIP

 PAGE \* MERGEFORMAT 8 On Determination of Ephedrine Theophylline compound theobromine content of the film [Abstract] Objective To establish the compound theobromine, theophylline ephedrine determination of the film. Methods using high performance liquid chromatography method with Kromasil C18 (4.6 mm × 250 mm, 5 μm) as the mobile phase was 0.05 mol / L potassium dihydrogen phosphate - methanol - triethylamine (77:23:0.2) and the detection wavelength was 215 nm, column temperature was 45 ℃, the flow rate was 0.8 mL / min. the results of theobromine in 0.309 4 ~ 0.722 μg (r = 0.999 96) within the concentration range of linear relationship between the average recovery was 100.49%, RSD = 1.1%. Conclusion The method is accurate, sensitive and rapid determination of theophylline, ephedrine tablets compound theobromine content. [Keywords:] compound theophylline ephedrine tablets, high performance liquid chromatography, theobromine, determination Abstract: Objective To establish a HPLC method for determination of theobromine in Compound Theophylline and Ephedrine Tablet. Method HPLC method was adopted on Kromasil C18 column (250 mm × 4.6 mm, 5 μm), and temperature was set at 45 ℃. The mobile phase consisted of 0.05 mol / L KH2PO4-Methanol-Triethanolamine (77:23:0.2), with flow rate of 0.8 mL / min. The UV detection wavelength was set at 215 nm. Results The calibration curves of theobromine was linear in the range of 0.3 094 ~ 0.722 μg (r = 0.999 96), ant the average recovery was 100.49% (n = 9) with RSD = 1.1%. Conclusion This method is simple, rapid, sensitive and accurate, and can be used for determination of theobromine in Compound Theophylline and Ephedrine Tablet. Keywords:: Compound Theophylline and Ephedrine Tablet, HPLC, theobromine, content determination Compound Theophylline and Ephedrine Tablets (formerly: compound theophylline tablets) is a cough and asthma compound preparation, test collection contained in the lt;”State Drug Standardsgt;” (