On Determination of quercetin in Apocynum tea.docVIP

 PAGE \* MERGEFORMAT 7 On Determination of quercetin in Apocynum tea [Abstract]: Objective To determine the content of quercetin in Apocynum tea by high performance liquid chromatography. Methods of methanol -0.4% phosphoric acid (50:50) as mobile phase Kromasil C18 column (250 mm × 4.6 mm , 5 μm), detection wavelength was 371 nm, column temperature was 30 ℃. Results Quercetin linear range 5.98 ~ 47.84 g / mL, the regression equation is A = 7 750.68C +1 196.33 (r = 0.999 7). Conclusions The method for accurate determination of quercetin in Apocynum tea. [Keywords:] quercetin in Apocynum tea HPLC determination Abstract: Objective To determine the content of quercetin in the Luobuma Tea by HPLC. Method The mobile phase consisted of methanol-0.4% phosphoric acid (50:50). Kromasil C18 column (250 mm × 4.6 mm, 5 μm) was used. The wavelength for measurement was at 371 nm and the temperature was at 30 ℃. Result The calibration curve was linear in the range of 5.98 ~ 47.84 g / mL for quercetin. The regression equation was A = 7 750.68C +1 196.33 (r = 0.999 7 ). Conclusion This method can be used to determine the content of quercetin in Luobuma Tea accurately. Keywords:: Luobuma Tea, quercetin, HPLC; content determination Apocynum Apocynum Apocynum venetum L. tea leaves are dried crushed single preparations made with Pinggan soothe the nerves, the role of heat diuresis. Clinically used for liver-yang, dizziness, palpitations, insomnia, edema, oliguria, hypertension, nerve weak, nephritis edema. Has been lt;lt;The Ministry of Health drug standard (traditional Chinese medicine prescription drugs)gt;gt; gathers, the standard required under the determination of rutin as reference, the use of spectrophotometry. In view of the determination method is more complicated, re- is poor, so choose the hydrolysis of aglycone quercetin rutin Apocynum tea as the determination of the control components. The test report Determination of quercetin in Apocynum tea methodolo