On the cytidine triphosphate on brain ischemia in rat brain-derived neurotrophic factor in the impact.docVIP

 PAGE \* MERGEFORMAT 6 On the cytidine triphosphate on brain ischemia in rat brain-derived neurotrophic factor in the impact Author: Li-Chen Wang Ru Wang Bing Dong Yujuan [Paper Keywords:] Brain-derived neurotrophic factor; cerebral ischemia; rat  [Abstract] Objective: To observe the CTP on the focal cerebral ischemia in rats BANF expression. Methods: Using immunohistochemical methods the effects of different ischemic time CTP treatment group and cerebral ischemia group the number of BDNF positive cells, and image analysis. Results: CTP group and ischemic group BDNF two cell morphology were not significantly different, but the number of positive cells CTP treatment group were significantly higher than the corresponding ischemic control group. Conclusion: cytidine triphosphate can improve cerebral ischemic penumbra the expression level of BDNF.  In recent years, studies have shown that cytidine triphosphate (Cytidine Triphosphate, CTP) as a promotion of nerve cell repair and regeneration of drugs. In this study, middle cerebral artery occlusion (middle cerebral artery occlusion, MCAO) model of focal cerebral ischemia CTP right brain-derived neurotrophic factor (brain-derived neurotrophic factor, BDNF) expression, in order to observe the CTP to the lack of blood plasticity of brain tissue damage.  1 Materials and Methods  SD rats were 96, were randomly divided into normal control group, sham operation group, ischemia group and CTP group. With internal carotid artery suture method of focal cerebral ischemia model. CTP Unit Application CTP intraperitoneal injection, dose 0.7mg/kg, once a day. Ischemia group and the CTP group were in ischemia 6h, 24h, 3d, 5d and 7d subjects, normal control group and sham-operated group and both the experiment after the start of day 7 derived, BDNF immunohistochemical staining using ABC method. Image Analysis: Take suprachiasmatic brain slices placed on the level of microscopic observation of 400 times the fiber, the