On the determination of CMV-specific antibody affinity and the diagnostic value of index.docVIP

 PAGE \* MERGEFORMAT 15 On the determination of CMV-specific antibody affinity and the diagnostic value of index Study: Lan Lin Pu Li Xin Niu Valley to learn English MA Xu [Abstract] Objective To fast, simple HCMV-specific antibody affinity index (CMV-IgG AI) detection method as an effective diagnostic tool to differentiate between primary CMV infection in pregnant women and non-primary infection. Methods Standard indirect ELISA method, 8.0 mol / L urea as a mild protein denaturant, with the standard curve method of quantitative determination of specimens in the CMV-IgG content. Results specimens of 218 cases were detected, of which 187 cases of CMV infection in past samples, 31 cases for the following issued or re-infection, suspected primary infection and primary infection samples. to antibody affinity index lt;35.0% as the positive standard, this time the sensitivity was 75.0%, specificity was 91.4%, 67.2% availability. previous infection, secondary or re-infection, suspected primary infection, primary infection, the average AI value of the 4 groups were (61.42 + -25.69)%, (70.38 + -21.88)%, (66.73 + -23.62)%, (29.78 ± 25.93)%, primary infection group and the other 3 groups of non-primary infection, the difference was statistically significant (P lt;0.01)). Conclusions 8.0 mol / L urea as a mild protein denaturant, with the standard curve method for quantitative determination CMV-IgG content of specimens for detection of cytomegalovirus IgG antibody affinity index (CMV-IgG AI), that the CMV-IgM detection combined CMV-IgG AI is to distinguish between primary and non-primary infection serological methods. [Keywords:] cytomegalovirus; affinity index; IgG antibody; Primary infection; enzyme-linked immunosorbent assay [Abstract] Objective To establish a rapid, easy and simple method for determination of CMV-IgG AI to serve as effective diagnostic tool to distinguish primary CMV infection from non-primary infection in pregnant women. Methods The measur