On the decoction at different boiling time TLC under.docVIP

 PAGE \* MERGEFORMAT 6 On the decoction at different boiling time TLC under [Paper Keywords] TLC; different boiling time In order to better choices boiling time so that the active ingredient decoction of the full fry up in order to give full play to efficacy. Hospital extracting machine to use my regular several decoction decoction decoction at different time, carried out by TLC, respectively, the results will now be briefly described below. Prescription 1: Poria 30 g, Guizhi 15 g, Atractylodes macrocephala 15 g, licorice 10 g, keel 50 g, Mu-grind 50 g, Chuanxiong 15 g, chrysanthemum 20 g, mint 10 g, Achyranthes 30 g, Tianma 20 g, magnet 30 g, Pinellia 20 g, Gegen 20 g, Prunella vulgaris 30 g. Indications: Hypertension: learn after 30 min decoction decoction 10 ml, concentrated to 1 ml, plus 70% methanol, 5 ml, ultrasonic treatment 30 min, filtration, the filtrate as a solution for the test items. Gastrodia control an alternative medicine 0.5 g, in contrast with the legal system, medicine solution. And then take gastrodin reference substance, add methanol produced per 1 ml solution containing 1 mg, as a reference substance solution. According to thin-layer chromatography test, drawing test items for the solution of the above 10 μ l, the control of various herbs, and reference substance solution 5 μ l, respectively, points on the same silica gel G thin-layer board, with ethyl acetate. Methanol. Water (9:1:0.2) as the agent to start out, dried, sprayed with 10% phosphomolybdic acid ethanol solution, heated at 105 ℃ to spot color definition. Chromatography products for the test with the control reference substance for medicinal and non-chromatographic position of the corresponding spots the same color. Learn from their experience after 60 min decoction decoction 10 ml, concentrated to 1 ml. The remaining steps Ibid. Test products for chromatography with the control reference substance for medicinal and the corresponding position chromatographic display the