Patients with hepatocellular carcinoma HepG2 hepatoma cells and dendritic cell fusion vaccine in vitro effects of.docVIP

 PAGE \* MERGEFORMAT 5 Patients with hepatocellular carcinoma HepG2 hepatoma cells and dendritic cell fusion vaccine in vitro effects of Author: Hong-Mei Zhang, Zhang Wang, Liu Chao, Bo-Rong Pan, Slovenia Xiaoming, Ren Jun [Keywords:] cell fusion Effect of a vaccine prepared by fusion of HepG2 cells with dendritic cells from patients with hepatocellular carcinoma in vitro [Abstract] AIM: To investigate the fusion ability of hepatocellular carcinoma (HCC) patientderived dendritic cells (DCs) with HCC cells (HepG2) in inducing autologous T lymphocytes to elicit specific immunity against HCC in vitro. METHODS: Peripheral blood mononuclear cells (PBMCs ) from HCC patients were isolated by blood separator. In the presence of recombinant human granulocyte / macrophageclone stimulating factor (rhGMCSF) and interleukin4 (rhIL4), PBMCs were cultured in vitro for 1 week to induce DCs. The expression of cell surface molecules was assessed by flow cytometry. The fusion cells of DCs with HepG2 cells (DCs/HepG2) were achieved by polyethylene glycol (PEG). The ability of DCs/HepG2 to stimulate the proliferation of autologous T lymphocytes was evaluated by MTT assay and the specific lysis of HepG2 by DCs/HepG2 induced cytotoxic T lymphocytes (CTLs) was detected by cytotoxicity test. RESULTS: After fusion, DCs/HepG2 highly expressed surface molecules, including CD83 90.4%, CD80 87.7%, CD86 84.4% and HLADR 98.5%. The fusion cells had a remarkably greater ability to stimulate the proliferation of autologous T lymphocytes in comparison with HepG2 and DCs. The DCs/HepG2activated CTLs showed a potent specific lysis to HepG2 cells, which was (63.5 ± 4.6)% with an effectortarget ratio 20:1 . CONCLUSION: The fusion of DCs derived from HCC patients with HepG2 cells can effectively stimulate autologous T lymphocytes to elicit specific antitumor immunity against HCC and may serve as a promising vaccine for immunotherapy of HCC. [Keywords] dendritic cells; cell fusion; carcinoma, hep