PDZ1 - 2 domains and recombinant adenovirus shuttle vector construction.docVIP

 PAGE \* MERGEFORMAT 14 PDZ1 / 2 domains and recombinant adenovirus shuttle vector construction Study: High-Fang, Hu book group, Sun Dong, Zhang Guangyi [Abstract] Objective To construct the domain of PSD-95 PDZ1 / 2 recombinant adenovirus. Ways to different guanidine sulfate cyanide - phenol - chloroform extraction step of total RNA as template, obtained by RT-PCR PDZ1 / 2 of the cDNA, with adenovirus shuttle vector with T4 DNA ligase, ligation products transformed into E. coli JM109 were screened, and sequenced. Results obtained by RT-PCR PDZ1 / 2 of the cDNA, enzyme digestion can