Penicillin-binding protein in Research.docVIP

 PAGE \* MERGEFORMAT 10 Penicillin-binding protein in Research Author: Helen Cha Yong Qian YAN Xiao-Ju Li Guang Xi Yu Yin river [Paper Keywords] deuterium marker of penicillin-binding protein polyacrylamide gel electrophoresis and fluorescence autoradiography [Abstract] At present, the growing problem of bacterial resistance to antibiotics, bring difficulties to the clinical treatment of β -lactam antibiotics in terms of certain strains of penicillin-binding proteins (PBPs) changes that result in drug resistance is a β -lactam antibiotics characteristics. General bacterial PBPs At present, the growing problem of bacterial resistance to antibiotics, to create difficulties for clinical treatment of ICI in terms of phthalocyanine amine antibiotics, some strains of penicillin-binding protein (PBps) changes that result in drug resistance is antibiotic ICI amine the characteristics of phthalate . General bacterial PBPs are cytoplasmic membrane proteins, is the target site bacterial death. It is the number, location, caused by changes in affinity with the ICI phthalocyanine amine combination antibiotic resistance is not easy. People often used SDS polyacrylamide gel electrophoresis and autoradiography method PBPs map, in order to understand the difference between resistant and susceptible strains. Therefore, PBPs pressure measurement technology is the study of drug resistance within the acid-amine an important means of [l]. Penetration weak, with a general law can not get autoradiography PBPs map requires the use of fluorescent flicker synergistic agents ‘fluorescence autoradiography’. Determination of technical reports relevant domestic PBps little to reporters less 3H standard, this article describes the technology. 1 Materials and methods 1.1 Materials 1.1.1 Strains of Streptococcus pneumoniae 31003,30301 (Beijing drugs and biological products; Pen08.R6 this room to save strain. 1.1.2 Medium C Y medium, see the literature (2). 1.1.3 Only devices and phar