Peripheral blood of patients with type 2 cell surface tissue factor expression and activity of.docVIP

 PAGE \* MERGEFORMAT 10 Peripheral blood of patients with type 2 cell surface tissue factor expression and activity of Study: Fine Lin Huang Yin Liu Lirong Tan Junfeng Shi Pengfei Qian Shen Quan Xie Yaping tall, thin yellow lotus [Keywords:] diabetes, tissue factor, thrombosis, mononuclear cells, platelets Type 2 diabetes mellitus (T2DM patients with thrombosis tendency in the formation and development of vascular disease plays a very important role. Recent studies have shown that thrombotic disease, not only in the level of organization, but at the cellular level has been in a hypercoagulable state (1) , tissue factor (TF is the initiating coagulation factors, in the past that comes mainly from vascular endothelial, and now found in peripheral blood mononuclear cells cells synthesized their own TF, platelets also contain TF (2), contains two kinds of cells after TF in T2DM patients with thrombosis in effect, little research at home and abroad. This paper examined the peripheral blood mononuclear cells in patients with T2DM and platelet membrane surface expression and activity of TF to explore their role in the pathogenesis of DM. 1 Subjects and methods 1.1 Object Diagnostic criteria by WHO DM (3) 42 patients diagnosed T2DM, 26 males and 16 females, aged 55 to 78 (mean (61.12 + -10.41 years). According to albumin excretion rate in DM group were divided into simple (DM group, 22 cases and combined DM microangiopathy (DN group, 20 cases. normal in 22 cases, male 12, female 10, aged 56 to 77 years (mean (63.52 + -11.21 years), excluding the thrombus disease and blood 2 w before used aspirin, heparin and other anticoagulant drugs. 1.2 Methods Flow cytometry Type: FACS Calibur, Becton Dickinson Company USA, CD62P PE-Cy5, CD61-PE, CD14 PE, and IgG2a isotype controls were purchased from BD Pharmingen company. TF FITC isotype control IgG1 and Spectrozyme FXa were purchased from American Diagnostica Inc. factor and factor a boatman purchased fr