Phosphatidylinositol -3 kinase signal transduction pathway in hypoxia-induced proliferation of human retinal pigment epithelial cells in the role of.docVIP

 PAGE \* MERGEFORMAT 23 Phosphatidylinositol -3 kinase signal transduction pathway in hypoxia-induced proliferation of human retinal pigment epithelial cells in the role of Author: Lan Lan, Wang Yu-sheng, Wang Yaochun, Zhang Peng Abstract of phosphatidylinositol -3 kinase (phosphatidylinositol 3-kinase, PI3K) in the hypoxia-induced human retinal pigment epithelial (retinal pigment epithelium, RPE) cell proliferation in the role. Methods: CoCl2 hypoxia model was established by the cultured cells. In hypoxic conditions, cultured human RPE cells, 1,4,8,12 and 24h, flow cytometry proliferative activity of RPE cells, RT-PCR and Western blot detection of phosphorylation of PI3K mRNA and protein expression levels. With 30μmol / L PI3K-specific inhibitor LY294002 treatment RPE cells, as a blocked experimental group. Results: With prolonged hypoxia, RPE cell proliferation activity was gradually increased phosphorylation of PI3K mRNA and protein expression levels gradually increased. LY294002 treatment group than in the control group RPE cell proliferation activity was significantly decreased (P lt;0.05). Theory: Hypoxia-induced RPE cell proliferation in part through the PI3K signal transduction pathway. Keywords: phosphatidylinositol 3 - kinase 0 Introduction In the age-related macular degeneration (age-related macular degeneration, AMD) are ignored, due to choroidal neovascularization (choroidal neovascularization, CNV) generated a series of secondary leakage, bleeding and scar formation and other changes, resulting in decreased visual acuity, or even lost. Experimental and clinical studies have shown that, CNV and choroidal the occurrence of local hypoxia on [1]. CNV occurrence in the process, the human retinal pigment epithelial (retinal pigment epithe-lium, RPE) cells play an important role in regulating. In hypoxic conditions, RPE cells by inducing the release of various cytokines, causing the degradation of the basement membrane