Phosphatidylinositol 3-kinase pathway in asthmatic rat airway smooth muscle cell proliferation role.docVIP

 PAGE \* MERGEFORMAT 13 Phosphatidylinositol 3-kinase pathway in asthmatic rat airway smooth muscle cell proliferation role Of: Wu Liqin, Daiyuan Rong, Xia Xiaodong [Abstract] Objective: To observe the lung tissue of asthmatic rats p-Akt, p-p70S6K levels, and explore the phosphatidylinositol 3 - kinase (PI3K signal transduction pathway in asthmatic rat airway smooth muscle cells (ASMC proliferation induced . Methods: SPF level 36 healthy male SD rats were randomly divided into control group (C group, asthma group (A group and wortmannin intervention group (W group, 12 in each group. ovalbumin (OVA sensitized and challenged the way rat model of chronic asthma. observe the airway inflammation in rats and cell infiltration, measured by image analysis software of airway wall thickness (Wat and airway smooth muscle layer (Wam, the Western blot, p-Akt in lung tissue and p-p70S6K expression. Results: A group Wat, Wam than C group (P lt;0.01, W rats Wat, Wam less than A group (P lt;0.01, greater than the C group (P lt;0.01. A group of p-Akt and p-p70S6K levels were higher than C group (P lt;0.01, W group than in A group (P lt;0.01, but higher than C group (P lt;0.01. p-Akt protein content and Wam / Pbm was a significant positive correlation (r = 0.779, P lt;0.01), p-Akt protein content and p-p70S6K protein content was significantly correlated (r = 0.803, P lt;0.01). Conclusion: The lung tissue of asthmatic rats PI3K/Akt / p70S6K pathway activation, inhibition of this pathway can reduce ASMC proliferation in asthmatic rats. [Keywords:] airway remodeling in asthmatic rats smooth muscle cell signal transduction Abstract: Objective: To observe the changes of p-Akt, p-p70S6K in lung tissues of asthma rat and to evaluate the role of phosphoinositide 3-kinase signal pathway in ASMCs proliferation in asthma. Methods: Thirty-six male Sprague-Dawley rats were randomly divided into three groups on average, including control group (group C), asthma group (group A) a