Pinellia protein on human hepatoma cells Bel 7402 Induction of apoptosis.docVIP

 PAGE \* MERGEFORMAT 13 Pinellia protein on human hepatoma cells Bel 7402 Induction of apoptosis 【Abstract】 Objective To observe and compare pinellin pairs of cultured human liver cancer cell growth inhibitory effect of Bel  7402. Methods Using flow cytometry to detect pinellin pairs of Bel  7402 human hepatocellular carcinoma cells induced apoptosis, and to observe their apoptosis-inducing effects of the amount of  effect relationship. The results of 60% ammonium sulfate precipitated protein Bel  7402 did not significantly induce apoptosis, while 30% ammonium sulfate precipitation protein can induce Bel  7402 cell apoptosis. 30% of the precipitation separation results further showed that only 0.1 mol / L NaCl elution peak have a certain Bel  7402 cells induced apoptosis. Conclusion pinellin a certain Bel  7402 cells induced apoptosis, the main ingredient that may exist in parts of the total organic acids. Keywords: Pinellia extract; human liver cancer cell Bel  7402; cell apoptosis Abstract: ObjectiveTo study the inducing apoptosis effects of Pinellia ternate protein on human hepatoma cell line bel  7402. MethodsFlow cytometry was used to test the effects of Pinellia ternate proteins. The dosage  effect relationship was also studied. ResultsThe 60% (NH4) 2SO4 depository protein showed no significant inducing apoptosis effects, while the 30% depository protein showed some effects, and 0.1 mol / L NaCl depository protein form 30% (NH4) 2SO4 deposition also showed some effects. Conclusion The Pinellia ternate protein has some effects of inducing apoptosis of Bel  7402, and probably the total organic acid is the main inducing apoptosis parts. Keywords:: Pinellia ternate protein; Human hepatoma cell line Bel  7402; Apoptosis Clinical Pinellia can be alone or with other drugs for the treatment of esophageal cancer compatibility, ovarian cancer and liver cancer. Anti-cancer traditional Chinese medicine in recent year