Plasma homocysteine serum folate and methylenetetrahydrofolate reductase gene polymorphism and colorectal cancer incidence Relationship.doc

 PAGE \* MERGEFORMAT 20 Plasma homocysteine serum folate and methylenetetrahydrofolate reductase gene polymorphism and colorectal cancer incidence Relationship [Abstract] Objective: To study the plasma homocysteine, serum folate and methylenetetrahydrofolate reductase (MTHFR) gene polymorphism and colorectal cancer incidence. Methods: Using PCR RELP 216 cases were detected colorectal cancer patients and 111 healthy people of the MTHFR C677T and A1298C polymorphisms of two loci, plasma homocysteine #8203;#8203;and serum folate levels were 96-well plates using HPLC method and microbiological assay, and its relationship with colorectal cancer. Results: MTHFR C677T of the CC, CT and TT in the gene in healthy controls the distribution of frequencies were 45.04%, 47.74% and 7.2%, colorectal cancer group was 40.74%, 47.2% and 12.03 % TT gene in case group was significantly higher (2 = 7.81, P lt;0.05). MTHFR A1298C of the AA and AC distribution of gene frequencies in the healthy control group were 45.04% and 54.95%, colorectal cancer group was 48.8 % and 53.12%, MTHFR A1298C allele frequency distribution in the two groups was no significant difference (2 = 0.56, P = 0.450 0). colorectal cancer gene in patients who have TT with the type of plasma total cysteine acid levels were significantly higher than CC and CT genes were carrying [respectively (16.12 + -9.03), (11.43 + -4.08) mol L-1, P = 0.002], TT gene in the control group were the same type of plasma total cysteine acid concentration was significantly lower than the CC and CT genes were carrying. colorectal cancer gene in patients who have TT levels of serum folate was significantly lower than CC and CT genes carry those [were (4.54 + -2.78), (6.86 + - 2.03) mg ml-1, P lt;0.05], gene in the control group TT serum folate concentrations were also significantly lower than the CC and CT genes were carrying. Conclusion: MTHFR C677T polymorphism and homocysteine low serum folate in colorectal cancer play a role in