Plasmodium falciparum apical membrane antigen 1 gene transfer model of Plasmodium berghei.doc

 PAGE \* MERGEFORMAT 10 Plasmodium falciparum apical membrane antigen 1 gene transfer model of Plasmodium berghei Author: Chen Jun, MIAO Jun, Liu Zhongxiang, Shu-Mei Li, Xue Cai-fang [Keywords:] Plasmodium berghei; Plasmodium falciparum; apical membrane antigen 1; transfection [Abstract] AIM: To establish the model of Plasmodium berghei (P. berghei) transfected with Plasmodium falciparum (P. falciparum) apical membrane antigen1 (AMA1) gene. METHODS: The transfection plasmid pDB.DTm.DB. / AB.AF. DB. containing P.falciparum AMA1 was enzymed by BglI. Blood stages of P.berghei were cultured in vitro, and mature schizonts were purified. Parasites transfected by electroporation were injected into tail veins of mice. Transfected parasites were selected by treating mice with pyrimethamine . DNA of transfected, resistant parasites was isolated and analyzed by PCR.RESULTS: The gene encoding P.falciparum AMA1 was amplified by PCR in the transfected parasites. CONCLUSION: The model of P.berghei transfected with P.falciparum AMA1 gene was established, which provided a basis for further study. [Keywords] Plasmodium berghei; Plasmodium falciparum; apical membrane antigen1; transfection [Abstract] Objective: To establish a Plasmodium falciparum Apical Membrane Antigen 1 (AMA1) gene transfer model of Plasmodium berghei. Methods: Plasmodium falciparum AMA1 containing the recombinant plasmid transfected pDB.DTm.DB. / AB.AF . DB. linearized with BglI digestion. berghei cultured in vitro and separation of power after the transformation, transformation of parasites after drug screening by PCR. Results: PCR test showed the existence transfected Plasmodium falciparum Plasmodium berghei AMA1 gene. Conclusion: The successful establishment of P. falciparum AMA1 gene transfection of Plasmodium berghei model provides a basis for further study. [Keywords:] Plasmodium berghei; Plasmodium falciparum; apical membrane antigen 1; transfection 0 Introduction Malaria is the world’s most se