Pleomorphic carcinoma of 6 cases of clinical pathology and immunohistochemical analysis of.docVIP

 PAGE \* MERGEFORMAT 14 Pleomorphic carcinoma of 6 cases of clinical pathology and immunohistochemical analysis of Keywords: Pleomorphic carcinoma of lung cancer Pathology Immunohistochemistry 0 Introduction Pleomorphic carcinoma of rare, poor prognosis, accounting for less than 1% of primary lung cancer [1,2]. In the new WHO classification of lung cancer, is a subtype of sarcomatoid carcinoma [1]. Histology showed malignant epithelial and homologous sarcomatoid spindle and / or giant cell components. Co-expression of keratin and vimentin contribute to the diagnosis of pleomorphic carcinoma. This paper reports six cases of pleomorphic carcinoma of the clinical, histological and immunohistochemical characteristics in order to explore this rare tumor pathological features and immunohistochemistry of its diagnostic role. 1 Data and methods 1.1 The clinical data of 6 cases in 5 males and 1 female. Age of onset from 49 to 76 years with a mean age of 63.33. 4 cases of age at onset over 60 years. The most common symptoms of cough (5 cases), chest pain (4 cases), hemoptysis (3 cases), fever (3 cases), there are two cases of fever or cough, chest X-ray examination showed lung mass. 4 cases of right lung, left lung in 2 cases. Lung upper lobe in 4 cases, the middle and lower lobe in 1 case. The average tumor size 5.08cm. Cases of 1,6 Ⅱ B period, cases of 2,4 Ⅲ A period, cases of 3 Ⅱ A period, cases of 5 Ⅲ B phase. 1.2 Pathology and immunohistochemistry 1.2.1 Pathology Methods 6 cases of pleomorphic lung cancer, surgical resection specimens in 10% neutral formalin-fixed, paraffin-embedded sections of normal, slice thickness 4μm, hematoxylin - eosin staining. 1.2.2 Immunohistochemical method: Select no obvious hemorrhage, necrosis, tissue fixation paraffin block as well to observe them. Wax sections, microwave antigen retrieval, immunohistochemistry with SP method, DAB color. Labeled antibody, including: CK7