PNP solution with detection precision of automatic biochemical analyzer.doc

 PAGE \* MERGEFORMAT 3 PNP solution with detection precision of automatic biochemical analyzer Author: Zhou Tie-Cheng, Xiao-Yun Yang, Chin Hing, Cheng Xiaodong, Xiao Peng Tao, Zhang Lu, YUAN Ya-juan, HAO Xiao-ke \ [Keywords:], pH indicator 0 Introduction At home and abroad have not yet introduced automatic biochemical analyzer precision detection of the standard method to study the reports and little. Nitrophenol (pNitrophenol, PNP) is an acid-base indicator, colorless to pale yellow crystal, soluble in water , which in aqueous solution with the pH change in the color change, color from colorless to yellow. We are in accordance with its color characteristics, with the PNP solution to do automatic analyzer precision wavelength of 340 nm detection kit, objectively reflect the equipment on a regular basis than the color system like the system working state and processing. good results reported as follows. 1 Materials and methods 1.1 Materials Automatic biochemical analyzer (HITACHI, 7170, Japan), UV-Vis spectrophotometer (UV2100, Shimadzu), PNP AR, Shanghai Chemical Reagents Center for chemical plant; pH 5.9, deionized water, pure water machines for the production of Hangzhou Tian Chuang. PNP working solution: Weigh 104.3 mg PNP, set in 500 mL of flask, dissolved in deionized water, and add water to the scale. 1.2 Methods PNP solution absorption spectrum of scan: According to PNP solution and deionized water to 300 mL/2700 mL mixing ratio will be placed in the mixture light path 1 cm quartz colorimetric cup to 690 nm to 190 nm absorption spectra of scanning, PNP solution for maximum absorption wavelength position. HITACHI, 7170 automatic biochemical analyzer precision testing process established by end-point method, the main wavelength of 340 nm, Vice-wavelength of 450 nm; reaction direction is positive; samples of deionized water, 10 mL, reagent I for the de-ionized water, 300 mL, reagent II for the 1.5 mmol / L PNP working solution 25 mL; measurement