Polygonum butanol extract on retinal ischemia-reperfusion injury in nitric oxide and nitric oxide synthase_0.docVIP

 PAGE \* MERGEFORMAT 14 Polygonum butanol extract on retinal ischemia-reperfusion injury in nitric oxide and nitric oxide synthase Of: Zhi-Hua Huang, Li Liangdong, Li Xiao, Zhang Chi, Xiong Jiao, Jing has been [Abstract] Objective Polygonum butanol extract (PBNA) on rat retinal ischemia-reperfusion injury in NO and NOS activity. Methods SD rats were randomly divided into 4 groups: sham operation group, retinal ischemia-reperfusion model group, low dose and high dose treatment group PBNA. sham operation group, after separation of the common carotid artery sublingual intravenous administration of 1 ml · kg-1 of the NS, other groups were in the common carotid artery occlusion, respectively, before the intravenous injection of physiological sublingual saline, low dose (0.3 mg · kg-1) and high dose (1 mg · kg-1) of the PBNA. common carotid artery occlusion and reperfusion 1 h 1 h after the electroretinogram (ERG) examination, blood, separation of serum, serum NO levels, T-NOS, iNOS and eNOS activity. Results Compared with the sham operation group, model group ERG amplitude decreased (P lt;0.01), serum NO content decreased (P lt;0.001), iNOS activity increased (P lt;0.05), eNOS activity decreased (P lt;0.05); with the model group, PBNA low dose NO content increased (P lt;0.05), iNOS activity decreased (P lt;0.05), low dose group and high ERG amplitude higher dose group (P lt;0.001), T-NOS (P lt;0.01) and eNOS (P lt;0.05, P lt;0.01) activity increased. Conclusion PBNA by improving T-NOS, and has a protective effect of eNOS activity, decreased with the injury of the activity of iNOS, increased NO levels, increased antioxidant capacity and vasodilator function, the protective effect on retinal function. [Keywords:] Polygonum butanol extract; retinal ischemia reperfusion injury; nitric oxide synthase Abstract: ObjectiveTo investigate the effect of Polygonum bistorta L. n-butyl alcohol (PBNA) extract on the NO content and NOS activity in ischemia / reperfusion