Polygonum capitatum Extraction of total flavonoids.docVIP

 PAGE \* MERGEFORMAT 10 Polygonum capitatum Extraction of total flavonoids Of: Zhao Chao, Bai, Wang book, Liuai Qin, Li Guoqing [Abstract] Objective Polygonum capitatum optimum extraction process of total flavonoids. Methods were optimized by orthogonal test method, Determination of total flavonoids. The results of ethanol concentration on the extraction of total flavonoids had significant effect. Conclusions and Orthogonal Experiment Results and analysis of variance to determine the amount of 12 times 55% ethanol extract 3 times, 1h / times for the best technology. [Keywords:] Polygonum capitatum, total flavonoids, extraction process, orthogonal Abstract: ObjectiveTo optimize the conditions for the extraction of the total flavonoids of Polygonum capitatum. MethodsThe optimum extraction process of the total flavonoids was selected by orthogonal experiment. The content of total flavonoids was determined by UV-spectrophotometry. ResultsSignificant effect of alcohol concentration is discovered. ConclusionThe optimum extraction process is as follows: Polygonum capitatum was extracted with 55% alcohol for 3 times, one hour per time, with the solvent volume 12 times amount . Keywords:: Polygonum capitatum; Total flavonoids; Extraction process; Orthogonal experiment Polygonum capitatum, alias Stone Mangcao, stone Polygonum and seasonal red, for the first species Polygonaceae Polygonum capitatum Polygonum capitatum Buch.-Ham. Ex D. Don dry the whole plant or above ground, mainly produced in Guizhou, Yunnan , Sichuan, Tibet, Guangxi and other provinces, with heat and dampness, detoxification and pain relief, and blood stasis, diuretic Tonglin and other effects. Polygonum capitatum has long been a folk herbal medicine, home and abroad for its chemical constituents, pharmacological effects of more less, the literature search, the main component of flavonoids [1,2], including benzene, formaldehyde, acetic acid, gallic acid, -sitosterol [3], antibacterial,