Polygonum multiflorum extract polyamide column chromatography flow sub-promoting activity of vascular endothelial cell growth.docVIP

 PAGE \* MERGEFORMAT 11 Polygonum multiflorum extract polyamide column chromatography flow sub-promoting activity of vascular endothelial cell growth [Abstract] Objective To investigate the extract of Polygonum multiflorum polyamide column chromatography flow points on the human umbilical vein endothelial cells proliferation. MTT colorimetric assay method of Polygonum multiflorum extract sub-flow polyamide column chromatography of human umbilical vein endothelial cell growth promoting effect; HE staining to observe the flow of sub-human umbilical vein endothelial cell morphology. Results by polyamide column chromatography eluted sub-fleece-flower root flow of human umbilical vein endothelial cells have different roles, including sub-D flow with increasing the concentration of human umbilical vein endothelial cells stimulated by under a microscope observed in the number of cells and an increase in split-phase, B, and C sub-stream little effect on cell proliferation, while the A sub-flow on vascular endothelial cells inhibit the growth of the role of reduction in the number of cells and nuclear pyknosis. Conclusion Polygonum multiflorum extract polyamide column chromatography, sub-containing stream to promote human umbilical vein endothelial cell growth substances. [Keywords:] Polygonum multiflorum polyamide column chromatography in human umbilical vein endothelial cells, MTT assay HE staining Abstract: ObjectiveTo investigate the proliferation effects on human umbilical vein vascular endothelial cells (HUVECs) of the fractions isolated from Polygonum multiflorm Thunb extracted with polyamide column chromatography. MethodsThe activity of promoting HUVECs growth of each fraction was observed by MTT colorimetric assay. The changes of HUVECs morphology were observed by HE stain. ResultsThe fractions eluted from polyamide column chromatography had different proliferation activities on HUVECs. With the increase of concentration, the fraction D could promote the gro