Polysaccharides and Dandelion ultrafiltration effects of free radical scavenging.doc

 PAGE \* MERGEFORMAT 11 Polysaccharides and Dandelion ultrafiltration effects of free radical scavenging Of: Xiao-Jie Yang, Chen, Cheng Jia-chun, LI Xue flowers [Abstract] Objective To study the polysaccharide ultrafiltration dandelion and free radical scavenging. Methods of ultrafiltration membranes with different pore sizes polysaccharide isolated dandelion, with the Fenton reaction system and the pyrogallol autoxidation method for the determination of hydroxyl radicals and superoxide anion scavenging. dandelion polysaccharide isolated ultrafiltration results of three components, DP1 and DP2 components measured free radical scavenging effect of the two components of the scavenging of hydroxyl radicals is strong, and higher than DP1 DP2, the two groups the effect of superoxide anion sub-obvious, DP1 slightly higher than the DP2. Conclusion membrane can be well separated dandelion polysaccharides, which a higher polysaccharide content DP2 component, accounting for 53.71%. DP1 and DP2 components can scavenge hydroxyl radicals and superoxide anion, hydroxyl radical scavenging ability, and higher than DP2 DP1, a concentration of 5 mg / ml when, DP2 clearance rate of 61.21%. [Keywords:] dandelion polysaccharide, ultrafiltration, hydroxyl radicals, superoxide anion Abstract: ObjectiveTo research on the ability to scavenge free radical of dandelion polysaccharide by ultrafiltration separation.MethodsDandelion polysaccharide were ultrafiltrated by ultrafiltration membrane of different aperture, and the scavenging effect on hydroxyl free radical and superoxide anion free radical were determined by Fenton system and the pyrogallol autoxidation method.ResultsThree parts were obtained by ultrafiltration membrane.The ability to scavenge free radical of DP1 and DP2 were determined.Two parts could scavenge hydroxyl free radical powerfully and DP2 had stronger activity than DP1.But the ability to scavange superoxide anion free radical of dandelion polyscccharide w