polβ high expression of the relevance of esophageal cancer cell drug resistance.doc

 PAGE \* MERGEFORMAT 6 polβ high expression of the relevance of esophageal cancer cell drug resistance [Keywords:] DNA polymerase β ; transfection; esophageal cancer; tumor cells, cultured; drug resistance, tumor; four methyl thiazolyl tetrazolium DNA polymerase beta (polymerase beta gene, polβ ) widely found in mammalian cell nucleus, is a Mr 39 * 103 single-peptide chain of small molecule protein, from 355 amino acids, is currently the smallest known DNA polymerase . In the base excision repair, DNA replication, translesion synthesis play an important role, but also to genomic instability related to [1-4]. In recent years, studies have shown that a variety of tumor tissues and tumor cell lines in polβ mRNA expression in are significantly increased [5-6]. polβ with the relationship between the drug resistance of tumor cells gradually attracted people’s attention. this experiment by studying the human esophageal cancer with cisplatin (concentration of cisplatin, cDDP)-resistant cell lines and stable high-Ec9706/cDDP Expression of human wild-type polβ the Ec9706 cells polβ expression level of its anti-tumor cDDP sensitivity to further explore the polβ high expression of the relevance of esophageal cancer cell drug resistance. 1 Materials and methods 1.1 Materials Human esophageal cancer cell line Ec9706 by the Chinese Academy of Medical Sciences Cancer Institute; cDDP Shandong Qilu Pharmaceutical Co., Ltd. products; RPMI 1640 medium Gibco / BRL Company production; four methyl thiazolyl tetrazolium (MTT) for the United States Sigma products; pEGFP  C3 (Clonetech Inc.); polβ wild-type human recombinant green fluorescent protein expression vector pEGFP  AC3 (Microbiology and Immunology Professor Zhao Guoqiang as gifts); G418 (Po biotech companies); 6  TG (USA Sigma Company); LipofectamineTM 2000 (Invitrogen Corporation); two pairs of primers from the Shanghai Health Industry Co., Ltd. Synthetic: polβ RNA detection primers: upstream 5’TGTTTGCCAGCTTC