Pomegranate husk of ursolic acid and oleanolic acid content by RP-HPLC.doc

 PAGE \* MERGEFORMAT 11 Pomegranate husk of ursolic acid and oleanolic acid content by RP-HPLC [Abstract] Objective To establish a RP-HPLC method for simultaneous determination of Pomegranate husk of ursolic acid and oleanolic acid content, and methodological study. Methods The analytical column was Kromasil C18 analytical column (4.6 mm * 250 mm, 5 μ m), mobile phase was methanol - water - phosphoric acid (88:12:0.1), flow rate 0.8 ml * min-1, photodiode array detector (PAD ), detection wavelength 210 nm, column temperature was 28 ℃ . Results Linear range of the selected chromatographic conditions were good, ursolic acid and oleanolic acid in the sample recoveries were 99.3% and 98.5%, RSD 2.20% and 1.78%. Conclusions were measured rapid, accurate, reliable, and can be used for Pomegranate husk of ursolic acid and oleanolic acid content in quantitative analysis. [Keywords:] Pomegranate husk high-performance liquid chromatography ursolic acid oleanolic acid photodiode array detector Abstract: ObjectiveTo develop a quantitative method for simultaneous determination of ursolic acid and oleanolic acid in Cortex Granati by RP-HPLC.MethodsHPLC with photodiode array detector (PAD) was carried out on Kromasil C18 column (4.6 mm * 250 mm, 5 μ m) at 28 ℃ using methanol-water-phosphoric acid (88:12:0.1) as mobile phase with a flow rate of 0.8 ml * min-1 and detected wavelength was at 210 nm.ResultsThe good linear relationship was obtained under the optimum conditions. The recoveries of the standards added for ursolic acid and oleanolic acid were 99.3% and 98.5%, respectively, while the relative standard deviations was 2.20% and 1.78%. ConclusionThe method is rapid and precise.It can be used to determine the ursolic acid and oleanolic acid in Cortex Granati. Keywords:: Cortex Granati; HPLC; Ursolic acid; Oleanolic acid; Photodiode array detector Pomegranate husk is a pomegranate pomegranate Punica granatum L. plants of the dry peel, Pickle, astringent, warm in nat