Postnatal rat visual cortex pyramidal neurons in the electrical properties.docVIP

 PAGE \* MERGEFORMAT 20 Postnatal rat visual cortex pyramidal neurons in the electrical properties Of: Meng Kai, Li Yanhai, Xie Wen, Zhang Li, Li Ping, Han too true [Abstract] Objective To investigate the postnatal rat visual cortex / layer of the electrical properties of pyramidal neurons. Methods Whole cell patch clamp combined with microscopic look into the cell technology, in acutely isolated brain slices of rat visual cortex specimens to explore the visual cortex of postnatal rats / layer pyramidal neurons and the electrical properties of passive and active action potential (AP) The release characteristics. Results after birth, 11 ~ 13d (P 11 ~ 13) in rats pyramidal neurons in the resting potential (-56.6 + -1.8) mV, input resistance (185.4 + -2.7) M, membrane capacitance (77.9 + -2.2) pF, the time constant (16.9 + -2.4) ms. AP amplitude and duration, respectively (97.7 + -2.7) mV and (2.3 + -0.1) ms, the threshold potential of (-31.8 + -1.4) mV, after hyperpolarization potential of (-65.3 + -1.3) mV. in the same by a long depolarizing current strength, the majority of neurons showed significant spike frequency adaptation. AP discharge frequency distribution about its stability when the first one discharge frequency (30.4 + -9.4)% . Conclusion P 11 ~ 13 rat visual cortex pyramidal neurons in the electrical properties not identical with the adult rats, most neurons exhibit firing pattern of the rules, but the firing rate of adaptation to a lesser extent. [Keywords:] electrical properties; rat; visual cortex; patch clamp ABSTRACT: Objective To investigate the electrophysiological properties of layer / pyramidal neurons of the early postnatal stage rat visual cortex.Methods By using the whole cell patch clamp recording technique combined with direct visualization of cells, we studied the passive and active electrophysiological properties as well as firing properties of layer / pyramidal neurons in acute rat visual cortical slices.Results R