PMA and calcium ionophore-induced K562 cells to dendritic cell differentiation.doc

 PAGE \* MERGEFORMAT 5 PMA and calcium ionophore-induced K562 cells to dendritic cell differentiation [Abstract] Objective To investigate the porphyrin alcohol myristate acetate (PMA) and calcium ionophore (CI)-induced K562 cells to dendritic cells (DC) differentiation role. Methods logarithmic phase of K562 cells with PMA and CI to cultivate the culture medium 96 h later, were observed under inverted phase contrast microscope, cell morphology, using flow cytometry phenotype was detected by MTT method to stimulate lymphocyte proliferation capacity. The results of a joint CI Unit PMA cell morphology changed significantly, the surface of many of the small processes, cell surface CD83, CD86, CD80, CD40, HLA  DR and CD1a expression increases, and can stimulate lymphocyte proliferation. Conclusion PMA Joint CI can be effective in differentiation of K562 cells to DC. [Keywords:] K562 cells in dendritic cells tetradecanoylphorbol acetate calcium ionophore ABSTRACT: Objective To explore the effect of PMA (phorbol myristate acetate) and calcium ionophore (CI) in inducing the differentiation of leukemia cell line K562 into activated dendritic cells (DC). Methods K562 cells were cultured in a common medium containing CI (A23187 ) and PMA for 96 h. The cell morphology was observed under the light phase contrast microscope and the electronic microscope. The viability rate of K562 cells cultured in each group was examined by the trypan blue staining. The immunologic phenotypes were analyzed by flow cytometry. The proliferation reaction of lymphocyte cells was detected by MTT colorimetry. Results After 96 h treatment with A23187 (375ug/ml) and PMA (10 μ g / mL), the cells exhibited the characteristic DC morphology, CD83, CD86, CD80, CD40 and HLA  DR, CD1a expression was upregulated and the cells were found to be able to strongly stimulate the proliferation of allogeneic T cells when cultured with them. Conclusion CI in combination with PMA can induce K562 cells to diff