Preconditioning induced by PKC PKC activation and vascular reactivity in hemorrhagic shock in rats and calcium sensitivity of the mechanism of protection.doc

 PAGE \* MERGEFORMAT 18 Preconditioning induced by PKC PKC activation and vascular reactivity in hemorrhagic shock in rats and calcium sensitivity of the mechanism of protection Of: Xu Jing, Li Tao, Yang Guangming, Liang-Ming Liu [Abstract] Objective To observe whether ischemic preconditioning through adenosine receptors induced protein kinase C @ (protein kinase C @, PKC @), protein kinase C (protein kinase C, PKC) activation and vascular reactivity in hemorrhagic shock rats and calcium sensitivity of protection. Methods Determination of adenosine during ischemic preconditioning concentration; using western blot technique to observe the A1, A2A, A2B, A3 adenosine receptors (A1, A2A, A2B, A3 adenosine receptor, A1R, A2AR, A2BR, A3R) inhibitors on ischemic preconditioning induced PKC @ and PKC expression and translocation of; tension in isolated microvascular measurement technique to observe the adenosine receptor inhibitor on ischemia preconditioning in hemorrhagic shock induced vascular reactivity and calcium sensitivity of protection. Results (1) 2 hours after shock compared with normal control plasma adenosine concentration was significantly increased, 5% of blood loss shock preconditioning can be further increased after the plasma adenosine concentration to 30 minutes before the shock and ischemic preconditioning group, the highest concentration of adenosine (P lt;0.01); (2) 5% blood loss 30 minutes before shock preconditioning may promote PKC @ and PKC from the cytoplasm to the cell membrane translocation (P lt;0.01), A1R antagonist significantly inhibited the ischemic preconditioning induced PKC @ and PKC translocation, so that PKC @ the membrane / cytosolic part of the ratio back down to 0.583 respectively 1.071, so that PKC in the membrane / cytosolic part of the ratio back down from the 1.280 0.606 (P lt;0.01), A2AR, A2BR and A3R antagonist had no obvious effect; (3) 5% blood loss 30 minutes before shock preconditioning induces vascular reactivit