Preparation of nano-demineralized bone matrix and properties testing.docVIP

 PAGE \* MERGEFORMAT 15 Preparation of nano-demineralized bone matrix and properties testing Of: Huang Kai, Chen Xiongsheng, JIA Shao will, Zhu Wei, Yan Wang Jun, the Housing Lei [Abstract] [Objective] MICROS nano superfine mill allogeneic demineralized bone matrix prepared (DBM), observe the nano-structure of DBM to study the nanocrystallization process of DBM as a bone graft substitutes biocompatibility. [ method] were prepared by modified Urist allogeneic demineralized bone matrix, frozen in liquid nitrogen grinding mill to block pre-DBM, the use of MICROS fine grind mill to further nano DBM. scanning electron microscope to observe the structure, according to the Ministry of Health lt; lt;biological materials and biological evaluation of medical equipment technical requirementsgt;gt; of the standards, the acute toxicity of nano-DBM, pyrogen test, hemolysis test and other testing. [results] Preparation of particle diameter of 50 ~ 200 nm demineralized bone matrix, biocompatibility testing non-toxic, non-heat resistance, do not cause hemolytic reaction, nano-DBM has good biocompatibility. [Conclusion] The nano-DBM control in low temperature conditions or preparation, is a non-toxic, no stimulation, without heat, without causing immune rejection of the biological material with good biocompatibility. [Keywords:] nano-demineralized bone matrix; MICROS superfine mill; microscopic characteristics; biocompatibility Abstract: [Objective] To observe the structure characteristics of nano decalcified bone matrix (DBM) produced by MICROS super fine mill, and to study the nano-technologies of DBM and the biocompatibility of nano-DBM as bone graft substitutes. [Methods] Improved Urist method was used to produce DBM. The pieces of DBM was pre-porphyrizated by liquid nitrogen frozen ball mill equipment. The DBM powder were further porphyrizated by MICROS super fine mill to be nano-DBM. The structure of nano-DBM was observed by electron microscope.The experiments