Preparation of solid-phase synthesis of cyclic peptide Hymenistatin1 and its analogs containing statine.docVIP

 PAGE \* MERGEFORMAT 18 Preparation of solid-phase synthesis of cyclic peptide Hymenistatin1 and its analogs containing statine [Abstract] Objective To study the solid-phase synthesis Preparation of Hymenistatin  1 [HS  1, sequence cyclo  ( Pro  Pro  Tyr  Val  Pro  Leu  Ile  Ile  )] and its analogues statine process steps and influencing factors. Methods Fmoc or Boc protected amino-α  to HBTU / NMM as condensing agent for straight-chain peptide in pick-peptide response to BOP / HOBt / DIEA as condensing agent for solid-phase synthesis cyclization HS  1 and its analogs. Chromatography and mass spectrometers used for the synthesis of cyclic peptide and its hybrid Purification and Identification of poly-peptide analogues. The results yield of peptide synthesis by 65% by reversed-phase high performance liquid chromatography (RP  HPLC) column of the synthetic peptides were purified by more than 90% purity by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI  MS) detection of the synthesis of cyclic peptide and cyclic peptide analogues of the molecular weight consistent with the theoretical molecular weight. Conclusion We successfully synthesized a high purity target peptide compounds. [Keywords:] solid-phase peptide synthesis; HS  1; Statine analogues; purification ABSTRACT Objective Preparation of Hymenistatin  1 [HS  1, cyclo  ( Pro  Pro  Tyr  Val  Pro  Leu  Ile  Ile  )] and its four statine  analogues by solid  phase peptide synthesis method, and the synthesis process and influencing factors were investigated. Methods Using the Fmoc  or Boc  protected α  amino acids, HBTU / NMM as coupling reagent (linear peptide synthesis) and BOP / HOBt / DIEA as cyclizing reagent, the cyclo  octapeptide HS  1 and its analogues were synthesized. The target peptides were purified by RP  HPLC and identified by MALDI  MS. Results The purity of the five peptides exceeded