Preparation of rat hind pairs of aging animal models of cervical disc degeneration.docVIP

 PAGE \* MERGEFORMAT 12 Preparation of rat hind pairs of aging animal models of cervical disc degeneration Wei-Ming Wang, Jin earth, Dong-Bin Qu 【Abstract】 Objective To amputated forelimb pairs of young rats to establish cervical disc degeneration animal model. Health 4-week-old male SD rats were 76, were randomly divided into two groups, surgery group 40, control group 36. Anesthesia after the surgery group were the roots of both upper limbs amputated limbs, respectively, after 3,6,9,12 months, were randomly divided into four groups, each group 10 rats were killed after the photo is being lateral cervical spine X-ray films, and the vertebral body specimens were cut preparation of C2 ~ C3 to T2 ~ T3 mid-sagittal histological sections, respectively HE, Safranin O staining OK disc tissue observed. Control animals, respectively, after the start of 4,8,12,16-month experiment were divided into four groups with the experimental group the same approach. Results after the cut-off addition to pairs of forelimbs, hind pairs of All rats survived the experimental rats after 9,12 months, suffered from cervical disc degeneration of the X-ray findings, light microscopy confirmed that the disc had occurred in the severe degeneration notochordal nucleus of fibrocartilaginous nucleus completely replaced with cartilage end-plate defects or disappeared, the matrix of collagen fibers arranged disorders, disc herniation, and ossification. Whereas the normal rats, only mild disc degeneration. Conclusion This modeling method does not damage target organs of normal animals, the anatomical structure of a high success rate, good repetition, in line with the human cervical intervertebral disc degeneration. 【Key Words】 disease model; animal; cervical intervertebral disc; X-ray; Histology 【Abstract】 Objective To develop a scientific and reproducible degenerative disc animal model for the study on the cervical disc.Methods bipedal rats model was developed in 40 infancy