Primary cultured neonatal rat sinoatrial node cells of morphology and surface antigen.doc

 PAGE \* MERGEFORMAT 19 Primary cultured neonatal rat sinoatrial node cells of morphology and surface antigen Of: management thinking Bin, Ma Ai-Qun, Jiang Wenhui [Abstract] Objective To establish a neonatal rat sinoatrial node cells (SNC) purified in vitro culture methods to understand the morphological characteristics of SNC’s observed surface antigen hyperpolarization-activated cyclic nucleotide gated cation channel gene 4 (HCN4) and gap connexin 45 (Cx45) expression characteristic of cells for cell biological pacemaker in-depth study to provide some experimental basis. Methods milk newborn SD rats sinoatrial node, successive trypsin digestion and differential adhesion to and 5 Br deoxyuridine (5 BrdU) for purification of sinoatrial node cells in primary culture. sinoatrial node results in cultured cells can be observed in 3 forms of cells, namely, spindle cells, triangular cells and polygonal cells of which the largest proportion of spindle cells, pulse frequency of the fastest developed organelles, few myofibrils, the expression of HCN4 and Cx45, the expression of triangular cells trace Cx45. CONCLUSION combination of trypsin digestion and differential adhesion 5 BrdU treatment, Purification of sinus node cells is a reliable culture, beating frequency fastest, HCN4 and Cx45 expression was positive in spindle cell lines may be pacemaker cells. [Keywords:] sinoatrial node cells cultured HCN4 ABSTRACT: Objective To understand morphological characteristics of sinoatrial node cells (SNCs) by taking purified isolation and culture of neonatal rat s SNCs in vitro and to understand the expression characteristics of surface antigens hyperpolarization activated cyclic nucleotide gated cation channel 4 (HCN4) and connexin 45 (Cx45) on SNCs. Methods The SNCs were isolated from sinus node tissues removed from neonatal rats by trypsin and purified cultured with differential attachment and 5 bromodeoxyuridine (BrdU) treatment. Results Three different types