Proanthocyanidins on lens epithelial cells induced by ultraviolet radiation protective effect of oxidative damage.doc

Proanthocyanidins on lens epithelial cells induced by ultraviolet radiation protective effect of oxidative damage [Abstract] Objective: To study the proanthocyanidins (procyanidins, PC) UV-induced human lens epithelial cells (len epithelial cells, LECs) DNA oxidative damage in rats. METHODS: The radiation dose 15mJ/cm2 of ultraviolet (UVB) were irradiated with 0 (control group), 0.05,0.1,0.2 mg / mL of the PC, taurine (TAU), vitamin C (VC) pretreatment of the LECs, and the untreated group did not give any anti-oxidant UV treatment, with single cell gel electrophoresis (SCGE) test to analyze the PC group the extent of LECs DNA single strand breaks, and with the other groups compared with the test results. Results: The PC group, tail length, percentage of tail DNA and tail moment in the numerical were significantly lower than the control group, compared with the control group, the difference was statistically significant (P lt;0.01). The PC end of the experimental group the percentage of DNA tail moment compared with the untreated group were not significantly different (Pgt; 0.05). the same concentration, PC, TAU and the VC group increases the detection index values. Conclusion: Exogenous PC UVB irradiation on human LECs DNA damage induced by a protective effect. PC is stronger protective effect at TAU, and VC, and in certain range of concentration with increasing concentration of the antioxidant effect gradually. [Keywords:] procyanidins; UV; lens epithelial cells; DNA damage AbstractAIM: To determine the protection of procyanidins (PC) against UVB induced oxidative damage of lens epithelial cells (LECs) with alkaline single cell gel electrophoresis, in order to provide an experimental foundation for cataract clinical treatment.METHODS: LECs lines of generation 4 were divided randomly into 5 groups. As untreated group: normal cultur ed LECs; control group: LECs + UVB; three treated groups: PC treated groups: LECs + PC + UVB; taurine (TAU) treat ed groups: LE