Proanthocyanidins on rat paw feet CIA tissue NFκ B-p65 protein and serum of certain pro-inflammatory cytokine expression in.doc

 PAGE \* MERGEFORMAT 8 Proanthocyanidins on rat paw feet CIA tissue NFκ B/p65 protein and serum of certain pro-inflammatory cytokine expression in Author: Lin Guan-ping, Liu Guoling, Ding, Liang Tong, ZHOU Ke-yuan [Abstract] Objective To study the procyanidins on type Ⅱ collagen-induced arthritis (CIA) rat paw claws of Nuclear Transcription Factor NF  κ B/p65 inhibition of protein expression and serum inflammatory cytokines IL  1β , TNF  α such as the level of impact. Methods of type Ⅱ collagen-induced rat model of CIA, immune staining foot claws tissue NF  κ B/p65 protein expression, ELISA assay CIA serum IL  1β and TNF  α content. The results of procyanidins dose-dependent manner reduced NF  κ B/p65 protein expression, reducing CIA serum TNF  α and IL  1β levels. Conclusion of procyanidins on the inhibition of inflammation in CIA in rats may be related to reduced NF  κ B/p65 protein expression, reducing pro-inflammatory cytokines TNF  α , IL  1β level of the. [Keywords:] Proanthocyanidins; Ⅱ collagen-induced arthritis; NF  κ B/p65; TNF  α ; IL  1β Abstract: Objective To investigate the inhibitory effect of proanthocyanidins on the expression of NF  κ B/p65 protein in the tissue of Type Ⅱ collagen  induced arthritis (CIA) rat’s paws, and the effects of proanthocyanidins on the contents of TNF  α , IL  1β in CIA rat’s serums.Methods CIA model in SD rats was established. The proteins expression of NF  κ B/p65 and MMP  9 in the skin of the rats paws was analyzed with immunohistochemistry. The contents of TNF  α and IL  1β in CIA rats serum were measured with ELISA.Results Proanthocyanidins can down  regulate the expression of NF  κ B / p65 protein and decrease level of TNF  α and IL  1β in CIA rats serums.Conclusion The anti  inflammatory mechanisms of proanthocyanidins may be related to decreasing the protein expression of NF  κ B/p65 and suppressing proinflammatory factors, ie, TNF 