Progress in DAT imaging technology and its application.docVIP

 PAGE \* MERGEFORMAT 14 Progress in DAT imaging technology and its application Keywords: DAT imaging; nuclear medicine; Parkinson’s disease Dopamine transporter (dopamine transporter, DAT) imaging part of the brain functional imaging, its initial development is based on Parkinson’s disease (Parkinson disease, PD) diagnosis. However, with the continuous development of nuclear medicine, DAT imaging is showing more and more significant value. 1 DAT structure and function of DAT is located in central dopamine (DA) neurons in the presynaptic membrane membrane protein, from about 620 amino acids, relative molecular mass of approximately 80 kD. DAT structure to 12 α-helical transmembrane region is characterized by, each transmembrane domain from 20 ~ 24 amino acid residues. DAT’s carboxyl-terminal and amino-terminal are located inside the cell. DAT amino sequence of noradrenaline (NA), 5 - hydroxytryptamine (5-HT), γ-aminobutyric acid (GABA) transporter, 40% ~ 60% homology, according to molecular structure and ion-dependent have been identified for DAT and NA ,5-HT and GABA transporters belong to the Na, Cl-dependent membrane transporter family [1]. In the DAT transmembrane domain of 12 hydrophobic transmembrane domains (transmenbrane domain, TMD) in, TMD-1 on the aspartic acid (Asp) is to determine the affinity with the size of DA binding sites, which with the DA on the The-NH 3 as ionic bonding. This point is also a DAT with certain compounds, such as drugs, addictive drugs, imaging agents interacting parts. TMD-7 on some of the serine residue of the catechol with the DA group interaction, to change these groups can significantly reduce the DAT and DA combination. DAT in the inner membrane amino acid sequence, inclusion of protein kinase C, cAMP-dependent protein kinase and calmodulin-dependent kinase phosphorylation sites, these sites of phosphorylation and dephosphorylation in the membrane can be controlled within a photo role a