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Progress in liver sinusoidal endothelial cells
PAGE \* MERGEFORMAT 28
Progress in liver sinusoidal endothelial cells
Keywords:: Liver sinusoidal endothelial cell research progress
Abstract This paper reviews the 90 years since the liver sinusoidal endothelial cells (HSEC) new progress in the study, including the HSEC the isolation and culture, the cell fenestrae and their associated adjustment factors, the first factor Ⅷ -related antigen expression and hepatic fibrosis the relationship between secretion of nitric oxide (NO) and endothelin (ET) and its impact on the liver microcirculation regulation and pathophysiological significance of a variety of cell surface receptors and adhesion molecules expression and function and its correlation with tumor metastasis and so on.
Liver sinusoidal endothelial cells (Hepatic Sinusoidal Endothelial cell, HSCE) is the liver nonparenchymal cells in the largest number of a cell, accounting for the total number of liver nonparenchymal cells by 50%. It is the formation of the structure and functional properties of vascular endothelial cells in general are very different. Vascular endothelial cell research in general are more extensive and in-depth earlier, HSEC studies are started late, in recent years, methods of isolation and culture with the HSEC continuous improvement of HSEC studies have also gradually increased and in-depth and found that HSEC activities in the liver function play an important role [1], this paper HSEC research progress in recent years are reviewed.
Isolation and culture of 1 HSEC
Endothelial cells in particular organ in the substantive study of vascular endothelial cells in vitro is dependent on many experimental methods. Knook for the first time with pronase (pronase) in liver tissue perfusion to centrifugal elutriation technique (centrifugal elutriation) Purification HSEC, but pronase can destroy the HSEC surface receptors, affect cell mass. Later replaced with pronase enterotoxin, enterotoxin only the destruction of liver cells, without destr
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