Promote the resumption of mouse bone marrow suppression associated factor IL7 Identification and cloning of genes.doc

 PAGE \* MERGEFORMAT 15 Promote the resumption of mouse bone marrow suppression associated factor IL7 Identification and cloning of genes Author: Shen Juan, HU Yao-hua, Shao Hongwei, Xiaolan Feng [Abstract] Objective To find ways of promoting recovery of bone marrow suppression related cytokines, bone marrow suppression when the differences in gene expression analysis, and increased expression of cytokines in the larger clone validation. Methods tail vein injection of 5  Fu build mouse model of bone marrow suppression, using gene chips to analyze the normal mice with bone marrow suppression model of the first 0,3,7,11,14-day differences in gene expression, increased expression of a larger mouse for IL  7 gene primers were designed to RT  PCR cloning of its coding sequence, and build phCMV / IL  7 eukaryotic expression vector. The results are consistent with the promotion of a group of cytokines characterized by recovery of bone marrow suppression genes, including cytokines IL  7 expression was increased 30 times. Conclusion IL  7 in line with the promotion of recovery of bone marrow suppression characteristics of cytokines for the study of recovery of bone marrow suppression laid the foundation for the new program. [Keywords:] Differential gene expression; IL  7; gene cloning; bone marrow suppression Abstract: Objective To find the cytokines that antagonized myelosuppression, and analyze gene expression in myelosuppressed mice and confirm remarkable upregulation of gene expression. Methods The myelosuppressed mice model was made by injection of 5  Fu in tail veils. The gene differential expression in day 0 , 3, 7, 11 and 14 respectively were analyzed with genechip. Specific primers of IL  7 which rose remarkably was designed to clone its full  length coding sequence (CDS) by RT  PCR, and then the eukaryotic vector phCMV / IL  7 was constructed. Results A group of cytokines that might play positive regulatory roles in suppression and