Proteasome inhibitor bortezomib in combination with daunorubicin on HL 60 cells apoptosis and the expression of Survivin mRNA.doc

 PAGE \* MERGEFORMAT 11 Proteasome inhibitor bortezomib in combination with daunorubicin on HL 60 cells apoptosis and the expression of Survivin mRNA [Abstract] Objective: To study the proteasome inhibitor bortezomib (Bor) alone or in combination with daunorubicin (DNR) on HL 60 cells and Survivin mRNA expression. Methods: Different concentrations of Bor, DNR and the two drug combination group and control group acts on HL 60 cells, using MTT cell proliferation, RT PCR detection of Survivin mRNA expression results: 5 nmol / L Bor for 24 h for the HL 60 cell proliferation, and induce their withered death. With the increasing concentration and treatment time can significantly increase the rate of apoptosis, 10 nmol / L Bor and 1.0 μmol / L DNR for 72 h, the joint highest rate of apoptosis, with the same dose with the two drugs alone significant than the difference (P lt;0.01). RT PCR results showed that: With the increase of drug concentration, Survivin mRNA expression decreased gradually, 10 mmol / L Bor and 1.0 μmol / L DNR combined effects of 72 h Survivin mRNA expression in the lowest Conclusion: Bor significantly inhibited HL 60 cell proliferation and induced apoptosis, Bor joint DNR on HL 60 cells, a synergistic effect, and significantly reduced the expression of Survivin gene, which may promote apoptosis of HL 60 cells of one. [Keywords:] proteasome inhibitor bortezomib daunorubicin HL 60 cells Abstract Objective: To investigate the effect of proteasome inhibitor bortezomib (Bor) alone and combination with daunomycin (DNR) on apoptosis of HL 60 cells and the expression of Survivin mRNA.Methods: HL 60 cells were treated with Bor, DNR in different concentration for 24 ~ 72 h.Cell proliferation was analyzed by MTT assay, the expression of survivin mRNA was analyzed by RT PCR.Results: 5 nmol / L Bor could effectively inhibit HL 60 cell proliferation and induced its apoptosis after 24 h.HL 60 cell apoptosis rate significantly increased with time prolong