Protease inhibitor on orthotopic liver transplantation in the pro-inflammatory cytokines and oxygen free radical metabolism.docVIP

 PAGE \* MERGEFORMAT 11 Protease inhibitor on orthotopic liver transplantation in the pro-inflammatory cytokines and oxygen free radical metabolism Author; FENG Guo-hui, LEI Zhi-li, Yu-peng, Ning Xinyu, Jiang Wei, Wang Tian-Tian, Li Jun [Abstract] Objective To evaluate the protease inhibitors, UTI (ulinary trypsin inhibitor, UTI) for orthotopic liver transplantation (orthotopic liver transplantation, OLT) in the plasma pro-inflammatory cytokines and free radicals level. Line will be 20 cases of elective surgery were randomly divided into two groups OLT. UTI group (U group, n = 10): skin incision after UTI 30 Wan u join 100ml saline, continuous infusion of 1h, and then repeat one time for every 4h. The control group (C group, n = 10): in order to replace the same volume of saline. After anesthesia, respectively, before skin incision (T0), no hepatic pre-120min (T1), anhepatic period of 30min (T2), new hepatic phase 5min (T3), new hepatic phase 60min (T4) and the completion of surgery (T5) collected venous Blood plasma IL-6, IL-8, TNF-α and malondialdehyde (MDA) concentration and superoxide dismutase (SOD) activity. Intraoperative continuous monitoring of cardiac output, mixed venous oxygen saturation, core temperature, ECG, CVP, PETCO2, SpO2, radial artery pressure, pulmonary arterial pressure. Maintain the core temperature of not less than 35.5 ℃. When the results of T0 between the two groups in plasma IL-6, IL-8, TNF-α, MDA, and SOD levels did not differ significantly (Pgt; 0.05). Compared with T0, both groups plasma IL-6, IL-8 from the T2 ~ T5 at different time points were higher (P lt;0.01); two groups of plasma TNF-α from the T1 ~ T5 at different time points were higher (P lt;0.05 or 0.01); two groups of plasma MDA from the T3 ~ T5 at different time points were higher (P lt;0.05 or 0.01); C plasma SOD from the T3 ~ T5 at different time points lower (P lt;0.01); U plasma SOD only in the T3, when lower (P lt;0.05). Compared with the C group, U plasma