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PRSV transgenic papaya 55-1 of the PCR detection of
PAGE \* MERGEFORMAT 12
PRSV transgenic papaya 55-1 of the PCR detection of
【Key Words】, transgenic; papaya 55-1;
Abstract: Objective To establish the fight against genetically modified papaya ringspot virus strain 55-1 to identify detection methods. Methods modified cetyltrimethyl ammonium bromide (CTAB) method and kit methods of papaya genomic DNA extraction, according to housekeeping Papain papaya ringspot virus genes and anti transgenic papaya lines 55-1 exogenous structural gene (35S-GUS) and control gene (NOS-35S) sequence-specific detection primers were designed and synthesized using PCR technology, against genetically modified papaya ringspot virus strain identified to 55-1. The results of endogenous gene PCR amplification results showed that the Papain, modified method of CTAB method and kit can be used for papaya seeds and pulp DNA extraction. Synthetic experiment of PCR primers and the establishment of the reaction system, reaction parameters can be specifically amplified transgenic papaya 55-1 exogenous 35S-GUS gene sequence and NOS-35S sequence. This method is the low absolute detection limit of 815 × 10-2ng, a relatively low detection limit of 014%. Conclusion This detection method has higher stability, which can effectively carry out strain of genetically modified papaya 55-1 identification, detection sensitivity of this method can completely satisfy the management of genetically modified food labeling qualitative detection needs.
Keywords:: transgenic; papaya 55-1;
PCRPCR for event-specific detection of transgenic virus resistant papaya 55-1
Abstract: Objective PCR assay was developed for event-specific detection of transgenic virus resistant papaya 55-1.Methods A developed Cetyl Trimethyl Ammonium Bromide (CTAB) method and Qiagen DNeasy plant mini kit were used to extract DNA from papaya seed and fruit.Specific primers for PCR detection of papaya 55-1 were designed to amplify endogenous papain gene sequence and exte
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