Purification of macroporous Long Sudo total flavonoids.docVIP

 PAGE \* MERGEFORMAT 11 Purification of macroporous Long Sudo total flavonoids Of: Xu Wei, Shu-Ling Zhang, Hong Zhenfeng, Zheng Hai Music [Abstract] Objective To study the AB 8 Separation and purification of a large dragon Sudo flavones. Desorption method by static adsorption experiments to screen the optimum resin, and the total flavonoid content of assessment indicators, the use of L9 (34 orthogonal method of AB 8 Separation and purification of a large dragon Sudo flavonoids investigated the factors to determine the optimal process conditions. Results The optimum conditions are: use of AB 8 macroporous resin, take Long sudo flavonoids extract (containing crude drug 0.5 g / mL) on the sample, the resin was 4 times the amount of crude drug, eluent 50% ethanol by volume fraction, the amount of 16 times the amount of crude drug. Conclusions The scientific and reasonable process conditions can be effectively used for long Sudo Flavonoids separation and enrichment. [Keywords:] Dragon sudo orthogonal design macroporous resin flavonoids Abstract: Objective To optimize the separation and purification procedure of total flavonoids from Bauhinia championii Benth by AB 8 macroporus resin. Methods The yield of total flavonoids was used as a maker . The static and dynamic adsorption / desorption methods and orthogonal design L9 (34were used to evaluate factors such as concentration of alcohol, ratio of alcohol to raw material and ratio of resin to raw material. The content of total flavonoids was determined by UV Vis spectrophotometer . Results The optimum conditions were as following: ratio of resin to raw material was 4, 50% of alcohol was used for eluent, ratio of alcohol to raw material was 16. Conclusion This procedure is reasonable, which could be used to separate and purify the total flavonoids from Bauhinia championii Benth. Keywords:: Bauhinia championii Benth, macroporus resin, orthogonal test, total flavonoids Long Sudo (Bauhinia championii Benth.