Purification of the process of camellia glucoside.doc

 PAGE \* MERGEFORMAT 9 Purification of the process of camellia glucoside [Abstract] [Objective] The study of macroporous resin for purification Camellia glycosides, optimize process conditions. [Method] to the adsorption rate, adsorption and desorption rate basis, the best screening macroporous resin. Using orthogonal design to transfer rate index, pH, adsorption time, the sample solution concentration, resin and test solution volume ratio on the macroporous resin adsorption. [results] YWD01G2 best resin. When the pH 6 ~ 7, time of 8h, the sample solution concentration 0.05g/mL, when the volume ratio of 1:1.5, macroporous resin adsorption for the best camellia glucoside. with 50% ethanol, you can get the product Camellia saponin content of 82.3%. [Conclusion ] macroporous resin treatment can significantly increase the Camellia glucosinolate content, the process simple and feasible. [Keywords:] resin, camellia glucoside, purification, process conditions Abstract: [Objective] To observe the effect of tea saponin purified by macro reticular resins, optimize the technique conditions. [Method] To screen the best macro reticular resin in terms of adsorption rate, capacity and desorption rate.Orthogonal test was used to observe the effect of pH, time, concentration and volume ratio of resin to solution on adsorption. [Result] YWD01G2 was best resin for adsorption.Its optimum conditions were pH6 ~ 7, time 8h, concentration 0.05g/mL, ratio 1:1.5. The saponin content reached 82.3% in elution of 50% ethanol. [Conclusion] Saponin content could be significantly promoted by macro reticular resin adsorption.The technique was brief and practicable. Keywords:: macro reticular resin; tea saponin; purification; technique conditions Camellia tea seed saponin was isolated from a mountain triterpenoid saponin compounds, it contains a hydrophilic molecular structure of sugar in the body and the hydrophobic ligand [1]. It is not only a natural non-ionic surfactant , wit